Application
Immunohistochemistry of POFUT1 in human heart tissue with POFUT antibody at 2.5 μg/mL.Application
Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | POFUT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POFUT1 Antibody: DDD2, FUT12, O-FUT, OFUCT1, O-Fuc-T, O-FucT-1, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1 |
| Product description | POFUT1 Antibody: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.1) Wang Y, Shao L, Shi S, et al. Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase. J. Biol. Chem.2001; 276:40338-45. |
| Immunogen | POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1. The immunogen is located within amino acids 90 - 140 of POFUT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| POFUT1 antibody can be used for the detection of POFUT1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POFUT1 |
|---|---|
| Alternative Names | POFUT1 Antibody: DDD2 FUT12 O-FUT OFUCT1 O-Fuc-T O-FucT-1 KIAA0180 GDP-fucose protein O-fucosyltransferase 1 Peptide-O-fucosyltransferase 1 |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2. |
Application
Immunohistochemistry of POFUT1 in human heart tissue with POFUT antibody at 2.5 μg/mL.
Application
Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | POFUT1 antibody can be used for the detection of POFUT1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | POFUT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | POFUT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
