Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with POLG2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of POLG2 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | POLG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA polymerase subunit gamma-2, mitochondrial, DNA polymerase gamma accessory 55 kDa subunit, p55, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, POLG2, MTPOLB |
| Product description | The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2).1) Longley,M.J., Am. J. Hum. Genet. 78 (6), 1026-1034 (2006) |
| Immunogen | This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-376 amino acids from the C-terminal region of human POLG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | POLG2 |
|---|---|
| Alternative Names | DNA polymerase subunit gamma-2 mitochondrial DNA polymerase gamma accessory 55 kDa subunit p55 Mitochondrial DNA polymerase accessory subunit MtPolB PolG-beta POLG2 MTPOLB |
| Molecular Weight(MW) | 55 kDa |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with POLG2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of POLG2 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
