Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | POLR1C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, RNA polymerases I and III subunit AC1, AC40, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RPA40, RPA39, RPC40, POLR1C, POLR1E |
| Product description | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft.1) Rush,J., Nat. Biotechnol. 23 (1), 94-101 (2005) |
| Immunogen | This POLR1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human POLR1C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | POLR1C |
|---|---|
| Alternative Names | DNA-directed RNA polymerases I and III subunit RPAC1 DNA-directed RNA polymerase I subunit C RNA polymerases I and III subunit AC1 AC40 DNA-directed RNA polymerases I and III 40 kDa polypeptide RPA40 RPA39 RPC40 POLR1C POLR1E |
| Molecular Weight(MW) | 39 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
