Application
Western blot analysis of POLR2D using rabbit polyclonal POLR2D Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).| Product Name | POLR2D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.1) Michiels, S., et al. Carcinogenesis 30(5):763-768(2009) |
| Immunogen | This POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the Central region of human POLR2D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR2D |
|---|---|
| Alternative Names | DNA-directed RNA polymerase II subunit RPB4 RNA polymerase II subunit B4 DNA-directed RNA polymerase II subunit D RNA polymerase II 16 kDa subunit RPB16 POLR2D |
| Molecular Weight(MW) | 16 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of POLR2D using rabbit polyclonal POLR2D Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.
Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
