Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | POLR2G Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.1) Cojocaru, M., et al. Biochem. J. 409(1):139-147(2008) |
| Immunogen | This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the C-terminal region of human POLR2G. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR2G |
|---|---|
| Alternative Names | DNA-directed RNA polymerase II subunit RPB7 RNA polymerase II subunit B7 DNA-directed RNA polymerase II subunit G RNA polymerase II 19 kDa subunit RPB19 POLR2G RPB7 |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
