Application
POLR2J Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | POLR2J Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.1) Michiels, S., et al. Carcinogenesis 30(5):763-768(2009) |
| Immunogen | This POLR2J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the C-terminal region of human POLR2J. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR2J |
|---|---|
| Alternative Names | DNA-directed RNA polymerase II subunit RPB11-a RNA polymerase II subunit B11-a RPB11a DNA-directed RNA polymerase II subunit J-1 RNA polymerase II 133 kDa subunit POLR2J POLR2J1 |
| Molecular Weight(MW) | 13 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
POLR2J Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
