Application
Confocal immunofluorescent analysis of PON2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
PON2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the detected PON2 protein (arrow).Application
Western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the detected PON2 protein (arrow).| Product Name | PON2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PON2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes.1) Sanghera, D.K., et al. Am. J. Hum. Genet. 62(1):36-44(1998) |
| Immunogen | This PON2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-101 amino acids from the Central region of human PON2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PON2 |
|---|---|
| Alternative Names | Serum paraoxonase/arylesterase 2 PON 2 Aromatic esterase 2 A-esterase 2 Serum aryldialkylphosphatase 2 PON2 |
| Molecular Weight(MW) | 39 kDa |
Application
Confocal immunofluorescent analysis of PON2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
PON2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the detected PON2 protein (arrow).
Application
Western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the detected PON2 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
