Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of POTE Antibody in Ramos cell line lysates (35ug/lane)| Product Name | POTE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The POTE protein contains seven ankyrin repeats between amino acids 140 and 380. Expression of POTE in prostate cancer and its undetectable expression in normal essential tissues make POTE a candidate for the immunotherapy of prostate cancer. The existence of a large number of closely related but rapidly diverging members, their location on multiple chromosomes and their limited expression pattern suggest an important role for the POTE gene family in reproductive processes.1) Bera T.K., Proc. Natl. Acad. Sci. U.S.A. 99:16975-16980(2002). |
| Immunogen | This POTE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-571 amino acids from the C-terminal region of human POTE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POTED |
|---|---|
| Alternative Names | POTE ankyrin domain family member D ANKRD26-like family B member 3 Ankyrin repeat domain-containing protein 21 Prostate ovary testis-expressed protein Protein POTE POTED A26B3 ANKRD21 POTE |
| Molecular Weight(MW) | 66 kDa |
| Entrez Gene | 100288966, 317754 |
|---|---|
| Protein Accession | Q86YR6 |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of POTE Antibody in Ramos cell line lysates (35ug/lane)| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
