Application
POU2F1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Product Name | POU2F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.1) Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010) |
| Immunogen | This POU2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-391 amino acids from the Central region of human POU2F1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU2F1 |
|---|---|
| Alternative Names | POU domain class 2 transcription factor 1 NF-A1 Octamer-binding protein 1 Oct-1 Octamer-binding transcription factor 1 OTF-1 POU2F1 OCT1 OTF1 |
| Molecular Weight(MW) | 76 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
POU2F1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
