POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes.1) Enomoto, Y., et al., (2004) Oncogene 23 (29), 5014-5022.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU2F3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
POU2F3 antibody can be used for detection of POU2F3 by ELISA at 1:1562500. POU2F3 antibody can be used for detection of POU2F3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Muscle.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
POU2F3 antibody can be used for detection of POU2F3 by ELISA at 1:1562500. POU2F3 antibody can be used for detection of POU2F3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU2F3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.