Application
Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 μg/mL.Application
Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 μg/mL.Application
Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | POU2F3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).1) Yukawa K, Yasui T, Yamamoto A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells. Gene 1993; 133:163-9. |
| Immunogen | POU2F3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human POU2F3. The immunogen is located within amino acids 370 - 420 of POU2F3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU2F3 |
|---|---|
| Alternative Names | POU2F3 Antibody: PLA1 OCT11 PLA-1 Epoc-1 OCT-11 OTF-11 Skn-1a OTF11 PLA1 POU domain class 2 transcription factor 3 Octamer-binding protein 11 41923 |
| Molecular Weight(MW) | Predicted: 48 kDa Observed: 55 kDa |
| Tissue Specificity | POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family. |
| Entrez Gene | 25833 |
|---|---|
| Protein Accession | NP_001231611 |
Application
Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 μg/mL.
Application
Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 μg/mL.
Application
Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | POU2F3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | POU2F3 antibody is supplied in PBS containing 0.02% sodium azide. |
