Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | POU3F3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]).[supplied by OMIM].1) Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006) |
| Immunogen | This POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human POU3F3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU3F3 |
|---|---|
| Alternative Names | POU domain class 3 transcription factor 3 Brain-specific homeobox/POU domain protein 1 Brain-1 Brn-1 Octamer-binding protein 8 Oct-8 Octamer-binding transcription factor 8 OTF-8 POU3F3 BRN1 OTF8 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Zebrafish |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
