POU3F4 (brain-specific homeobox/POU domain protein 4, brain-4, Brn-4, transcription factor 4) is a transcription factor with a POU domain.1) Xia, A.P., et al., (2002) Hear.Res.166(1-2), 150-158.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU3F4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
POU3F4 antibody can be used for detection of POU3F4 by ELISA at 1:1562500. POU3F4 antibody can be used for detection of POU3F4 by western blot at 0.3-0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.3-0.5 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
POU3F4 antibody can be used for detection of POU3F4 by ELISA at 1:1562500. POU3F4 antibody can be used for detection of POU3F4 by western blot at 0.3-0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU3F4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL.