Application
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.Application
Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PPAPDC1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPAPDC1A Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis.1) Takeuchi M, Harigai M, Momohara S, et al. Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase. Gene2007; 399:174-80. |
| Immunogen | PPAPDC1A antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PPAPDC1A. The immunogen is located within the last 50 amino acids of PPAPDC1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPAPDC1A |
|---|---|
| Alternative Names | PPAPDC1A Antibody: DPPL2 PPAPDC1 DPPL2 Phosphatidate phosphatase PPAPDC1A Phosphatidic acid phosphatase type 2 domain-containing protein 1A |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Entrez Gene | 196051 |
|---|---|
| Protein Accession | NP_001025230 |
Application
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.
Application
Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PPAPDC1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PPAPDC1A Antibody is supplied in PBS containing 0.02% sodium azide. |
