Application
Immunofluorescence of PPAPDC2 in Raji cells with PPAPDC2 antibody at 20 μg/mL.Application
Western blot analysis of PPAPDC2 in Raji cell lysate with PPAPDC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PPAPDC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPAPDC2 Antibody: PPAPDC2 is a phosphatase that dephosphorylates Presqualene diphosphate (PSDP) into presqualene monophosphate (PSMP), suggesting that it may have important role in innate immunity. PSDP is a bioactive lipid that rapidly remodels to PSMP upon cell activation. PPAPDC2 displays diphosphate phosphatase activity with a substrate preference for PSDP > FDP > phosphatidic acid. PPAPDC2 activity is independent of Mg2+ and has been identified in activated human neutrophil (PMN) extracts. It is widely expressed in human tissues. Recent studies shows PPAPDC2 is a functional isoprenoid diphosphate phosphatase.1) Fukunaga K, Arita M, Takahashi M, et al. Identification and functional characterization of a presqualene diphosphate phosphatase. J. Biol. Chem.2006; 281:9490-7. |
| Immunogen | PPAPDC2 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus human PPAPDC2. The immunogen is located within amino acids 170 - 220 of PPAPDC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PPAPDC2 antibody can be used for detection of PPAPDC2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPAPDC2 |
|---|---|
| Alternative Names | PPAPDC2 Antibody: PDP1 PSDP bA6J24.6 Presqualene diphosphate phosphatase Phosphatidic acid phosphatase type 2 domain-containing protein 2 PPAP2 domain-containing protein 2 |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
Application
Immunofluorescence of PPAPDC2 in Raji cells with PPAPDC2 antibody at 20 μg/mL.
Application
Western blot analysis of PPAPDC2 in Raji cell lysate with PPAPDC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | PPAPDC2 antibody can be used for detection of PPAPDC2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PPAPDC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PPAPDC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
