Application
Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of PPAPDC3 in mouse heart tissue with PPAPDC3 antibody at 20 μg/mL.| Product Name | PPAPDC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPAPDC3 Antibody: PPAPDC3, also known as nuclear envelope transmembrane protein 39 (NET39), was initially discovered in an in silico screen for secreted or membrane proteins. It is a member of the PAP2 superfamily of phosphatases and haloperoxidases. PPAPDC3 has recently been shown to act as a negative regulator of myoblast differentiation by diminishing the activity of the mammalian target of rapamycin TOR. PPAPDC3 is highly expressed in cardiac and skeletal muscle and becomes strongly upregulated during cultured myoblast differentiation tissues. Overexpression of PPAPDC3 in myoblasts repressed myogenesis while knockdown by RNA interference promoted differentiation indicating its part in the regulatory mechanism for myogenesis.1) Otsuki T, Ota T, Nishikawa T, et al. Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res.2005; 12:117-26. |
| Immunogen | PPAPDC3 antibody was raised against a 19 amino acid synthetic peptide from near the amino of terminus human PPAPDC3. The immunogen is located within the first 50 amino acids of PPAPDC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PPAPDC3 antibody can be used for detection of PPAPDC3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPAPDC3 |
|---|---|
| Alternative Names | PPAPDC3 Antibody: NET39 C9orf67 Probable lipid phosphate phosphatase PPAPDC3 Phosphatidic acid phosphatase type 2 domain-containing protein 3 |
Application
Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of PPAPDC3 in mouse heart tissue with PPAPDC3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | PPAPDC3 antibody can be used for detection of PPAPDC3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PPAPDC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PPAPDC3 Antibody is supplied in PBS containing 0.02% sodium azide. |
