Application
Western blot analysis of lysed extracts from HeLa, 293, 3T3 and MCF-7 cells using PPARγ Antibody.| Product Name | PPARgamma Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.1) "Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARgamma2 versus PPARgamma1 and activation with retinoid X receptor agonists and antagonists. "Mukherjee R., Jow L., Croston G. E., et al. J. Biol. Chem. 272:8071-8076 (1997) "Cloning and characterization of RAP250, a nuclear receptor coactivator. "Caira F., Antonson P., Pelto-Huikko M., et al. J. Biol. Chem. 275:5308-5317 (2000) "Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha. "Qi C., Chang J., Zhu Y., et al. J. Biol. Chem. 277:28624-28630 (2002) |
| Immunogen | PPARgamma antibody was raised against a peptide sequence around aa.50~54 (L-S-V-M-E) derived from Human PPARγ. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPARG |
|---|---|
| Alternative Names | GLM1 CIMT1 NR1C3 PPARG1 PPARG2 PPARgamma MAPTL MTBT1 TAU GLM1 PPARG |
| Molecular Weight(MW) | 53 57 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total PPARγ protein. |
| Entrez Gene | 5468 |
|---|---|
| Protein Accession | NP_005028.4 |
Application
Western blot analysis of lysed extracts from HeLa, 293, 3T3 and MCF-7 cells using PPARγ Antibody.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
