Application
Immunohistochemistry of PPARGC1A in human liver tissue with PPARGC1A antibody at 5 μg/mL.Application
Immunofluorescence of PPARGC1A in human liver tissue with PPARGC1A antibody at 20 μg/mL.Application
Western blot analysis of PPARGC1A in mouse liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.| Product Name | PPARGC1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).1) Tsuemi T and La Spada AR. PGC-1a at the intersection of bioenergetics regulation and neuron function: from Huntington’s disease to Parkinson’s disease and beyond. Prog. Neurobiol. 2012; 97:142-51. |
| Immunogen | PPARGC1A antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PPARGC1A. The immunogen is located within the last 50 amino acids of PPARGC1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| PPARGC1A antibody can be used for detection of PPARGC1A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPARGC1A |
|---|---|
| Alternative Names | PPARGC1A Antibody: LEM6 PGC1 PGC1A PGC-1v PPARGC1 PGC-1(alpha) LEM6 Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Ligand effect modulator 6 PGC-1-alpha |
| Molecular Weight(MW) | Predicted: 88 kDa Observed: 96 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform. |
Application
Immunohistochemistry of PPARGC1A in human liver tissue with PPARGC1A antibody at 5 μg/mL.
Application
Immunofluorescence of PPARGC1A in human liver tissue with PPARGC1A antibody at 20 μg/mL.
Application
Western blot analysis of PPARGC1A in mouse liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | PPARGC1A antibody can be used for detection of PPARGC1A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PPARGC1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide. |
