Application
Antibody used in WB on Human, Hamster at 1:5000.Application
Antibody used in WB on Human HepG2 at 0.25 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | PPAT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.1) Bera, A.K., (1999) J. Biol. Chem. 274 (51), 36498-36504. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PPAT antibody can be used for detection of PPAT by ELISA at 1:62500. PPAT antibody can be used for detection of PPAT by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPAT |
|---|---|
| Alternative Names | PPAT ATASE GPAT PRAT |
| Molecular Weight(MW) | 56 kDa |
Application
Antibody used in WB on Human, Hamster at 1:5000.
Application
Antibody used in WB on Human HepG2 at 0.25 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PPAT antibody can be used for detection of PPAT by ELISA at 1:62500. PPAT antibody can be used for detection of PPAT by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPAT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
