Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Product Name | PPCDC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine.1) Daugherty, M., (2002) J. Biol. Chem. 277 (24), 21431-21439. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPCDC. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PPCDC antibody can be used for detection of PPCDC by ELISA at 1:312500. PPCDC antibody can be used for detection of PPCDC by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPCDC |
|---|---|
| Alternative Names | PPCDC FLJ14585 MDS018 |
| Molecular Weight(MW) | 22 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PPCDC antibody can be used for detection of PPCDC by ELISA at 1:312500. PPCDC antibody can be used for detection of PPCDC by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPCDC antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
