Application
Western blot analysis in T47D cell line lysates (35ug/lane).Application
PPL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PPL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq].1) Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) : |
| Immunogen | This PPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1330 amino acids from the C-terminal region of human PPL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPL |
|---|---|
| Alternative Names | Periplakin 190 kDa paraneoplastic pemphigus antigen 195 kDa cornified envelope precursor protein PPL KIAA0568 |
| Molecular Weight(MW) | 205 kDa |
Application
Western blot analysis in T47D cell line lysates (35ug/lane).
Application
PPL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
