WB
Various lysates were subjected to SDS PAGE followed by western blot with PPL antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using PPL antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PPL antibody at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).| Product Name | PPL Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PPL protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:800 ICC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPL |
|---|---|
| Gene Full Name | periplakin |
| Gene Summary | The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq, Jul 2008] |
| Alternative Names | periplakin KIAA0568 |
| Molecular Weight(MW) | 205kDa |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Cytoskeleton,Membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PPL antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using PPL antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PPL antibody at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:800 ICC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
