Application
Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 μg/ml.Application
Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 μg/ml.Application
Western blot analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 μg/ml.| Product Name | PPM1D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein phosphatase Mg2+/Mn2+ dependent 1D, p53-induced protein phosphatase 1, PP2C-DELTA, Protein phosphatase 1D, WIP1 |
| Product description | Wild-type p53 induced phosphatase 1 (WIP1) / protein phosphatase magnesium-dependent 1 delta (PPM1D), a protein identified in the p53 DNA response pathway, is a member of the PP2C family of serine/threonine protein phosphatases which selectively inactivates p38 MAPK and dephosphorylates the ATM/ATR targets, Chk1 and p53 (1,2). Studies have shown that it is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (2,3). PPM1D deletion results in a reduction of T and B cell function and compromised cell division, rendering cells resistant to becoming cancerous and slowing tumor development (4,5).1) Fiscella M, Zhang H, Fan S, et al. Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner. Proc. Natl. Acad. Sci. USA. 1997; 94:6048-53. |
| Immunogen | PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D. The immunogen is located within the last 50 amino acids of PPM1D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPM1D |
|---|---|
| Alternative Names | Protein phosphatase Mg2+/Mn2+ dependent 1D p53-induced protein phosphatase 1 PP2C-DELTA Protein phosphatase 1D WIP1 |
| Molecular Weight(MW) | Predicted: 67 kDa Observed: 68 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform. |
Application
Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 μg/ml.
Application
Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 μg/ml.
Application
Western blot analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PPM1D antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PPM1D antibody is supplied in PBS containing 0.02% sodium azide. |
