Application
Antibody used in WB on HEK293, H1299 at 1:1000 (Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate ).Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | PPP1CA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Lesage, B., (2007) Biochemistry 46 (31), 8909-8919. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPP1CA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PPP1CA antibody can be used for detection of PPP1CA by ELISA at 1:1562500. PPP1CA antibody can be used for detection of PPP1CA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP1CA |
|---|---|
| Alternative Names | PPP1CA MGC15877 MGC1674 PP-1A PPP1A PP1A PP1alpha |
| Molecular Weight(MW) | 38 kDa |
| Entrez Gene | 5499 |
|---|---|
| Protein Accession | NP_001008709 |
Application
Antibody used in WB on HEK293, H1299 at 1:1000 (Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate ).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PPP1CA antibody can be used for detection of PPP1CA by ELISA at 1:1562500. PPP1CA antibody can be used for detection of PPP1CA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPP1CA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
