Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | PPP1R3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein phosphatase 1 regulatory subunit 3A, Protein phosphatase 1 glycogen-associated regulatory subunit, Protein phosphatase type-1 glycogen targeting subunit, RG1, PPP1R3A, PP1G |
| Product description | The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq].1) Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009) |
| Immunogen | This PPP1R3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-782 amino acids from the Central region of human PPP1R3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP1R3A |
|---|---|
| Alternative Names | Protein phosphatase 1 regulatory subunit 3A Protein phosphatase 1 glycogen-associated regulatory subunit Protein phosphatase type-1 glycogen targeting subunit RG1 PPP1R3A PP1G |
| Molecular Weight(MW) | 126 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rb |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
