Application
Western Blot at 1:2000 dilution Lane 1: BA/F3 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PPP2R4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.1) Cayla X.,et al.J. Biol. Chem. 269:15668-15675(1994). |
| Immunogen | This PPP2R4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-35 amino acids from human PPP2R4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP2R4 |
|---|---|
| Alternative Names | Serine/threonine-protein phosphatase 2A activator 5.2.1.8 PP2A subunit B' PR53 isoform Phosphotyrosyl phosphatase activator PTPA Serine/threonine-protein phosphatase 2A regulatory subunit 4 Serine/threonine-protein phosphatase 2A regulatory subunit |
| Molecular Weight(MW) | 41 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: BA/F3 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
