Application
Western blot analysis of anti-PPP3CC Pab in A375cell line lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PPP3CC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Calmodulin-dependent protein phosphatase, calcineurin, is involved in a wide range of biologic activities, acting as a Ca(2+)-dependent modifier of phosphorylation status. In testis, the motility of the sperm is thought to be controlled by cAMP-dependent phosphorylation and a unique form of calcineurin appears to be associated with the flagellum. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. At least 3 genes, calcineurin A-alpha, calcineurin A-beta, and calcineurin A-gamma, have been cloned for the catalytic subunit. These genes have been identified in humans, mice, and rats, and are highly conserved between species (90 to 95% amino acid identity).1) Eastwood, S.L., et al., Biol. Psychiatry 57(7):702-710 (2005). |
| Immunogen | This PPP3CC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-512 amino acids from the C-terminal region of human PPP3CC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PPP3CC |
|---|---|
| Alternative Names | Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform CAM-PRP catalytic subunit Calcineurin testis-specific catalytic subunit Calmodulin-dependent calcineurin A subunit gamma isoform PPP3CC CALNA3 CNA3 |
| Molecular Weight(MW) | 58 kDa |
Application
Western blot analysis of anti-PPP3CC Pab in A375cell line lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
