Application
Western blot analysis of MAPKAPK5 using rabbit polyclonal MAPKAPK5 Antibody (T182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PRAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase, PRAK, MAPKAPK5, PRAK |
| Product description | MAPKAPK5 is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.1) New, L., et al., EMBO J. 17(12):3372-3384 (1998). |
| Immunogen | This PRAK(MAPKAPK5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from human PRAK(MAPKAPK5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPKAPK5 |
|---|---|
| Alternative Names | MAP kinase-activated protein kinase 5 MAPK-activated protein kinase 5 MAPKAP kinase 5 MAPKAP-K5 MAPKAPK-5 MK-5 MK5 p38-regulated/activated protein kinase PRAK MAPKAPK5 PRAK |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis of MAPKAPK5 using rabbit polyclonal MAPKAPK5 Antibody (T182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.
Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
