Application
Western blot analysis using PRAK monoclonal antibody against truncated PRAK - His recombinant protein.Application
Immunohistochemical analysis of paraffin - embedded human normal spleen (top) and human breast carcinoma (bottom) showing nuclear / cytoplasma location using PRAK antibody with DAB staining.| Product Name | PRAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRAKMAPK-activated protein kinase 5, MK5, MK-5, PRAK, MAPKAP-K5 |
| Product description | PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.1) Paliga AJ. Natale DR. Watson AJ. Biol Cell. 2005, Aug, 97(8):629-40. |
| Immunogen | Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAPKAPK5 |
|---|---|
| Alternative Names | PRAKMAPK-activated protein kinase 5 MK5 MK-5 PRAK MAPKAP-K5 |
Application
Western blot analysis using PRAK monoclonal antibody against truncated PRAK - His recombinant protein.
Application
Immunohistochemical analysis of paraffin - embedded human normal spleen (top) and human breast carcinoma (bottom) showing nuclear / cytoplasma location using PRAK antibody with DAB staining.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRAK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
