Application
Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 μg/mL.Application
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 μg/mL.Application
Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.| Product Name | PRC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRC1 Antibody: ASE1, Protein regulator of cytokinesis 1 |
| Product description | PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.1) Jiang W, Jimenez G, Wells NJ, et al. PRC1: a human mitotic spindle-associated Cdk substrate protein required for cytokinesis. Mol. Cell 1998; 2:877-85. |
| Immunogen | PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1. The immunogen is located within amino acids 350 - 400 of PRC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRC1 |
|---|---|
| Alternative Names | PRC1 Antibody: ASE1 Protein regulator of cytokinesis 1 |
| Tissue Specificity | At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Application
Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 μg/mL.
Application
Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
