Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | PRDM6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression (By similarity).1) Rose, J. Phd, et al. Mol. Med. (2010) In press : |
| Immunogen | This PRDM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-72 amino acids from the N-terminal region of human PRDM6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRDM6 |
|---|---|
| Alternative Names | Putative histone-lysine N-methyltransferase PRDM6 PR domain zinc finger protein 6 PR domain-containing protein 6 PRDM6 PFM3 |
| Molecular Weight(MW) | 64 kDa |
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
