Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PRDX6 Antibody in CEM cell line lysates (35ug/lane)Application
Western blot analysis of PRDX6 using rabbit polyclonal PRDX6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2).| Product Name | PRDX6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PRDX6is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.1) Sorokina,E.M., et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 297 (5), L871-L880 (2009) |
| Immunogen | This PRDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-224 amino acids from the C-terminal region of human PRDX6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PRDX6 |
|---|---|
| Alternative Names | Peroxiredoxin-6 1-Cys peroxiredoxin 1-Cys PRX 24 kDa protein Acidic calcium-independent phospholipase A2 aiPLA2 311- Antioxidant protein 2 Liver 2D page spot 40 Non-selenium glutathione peroxidase NSGPx Red blood cells page spot 12 PRDX6 AOP2 |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PRDX6 Antibody in CEM cell line lysates (35ug/lane)
Application
Western blot analysis of PRDX6 using rabbit polyclonal PRDX6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
