Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
PREX1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PREX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 3,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,5)-dependent Rac exchanger 1, PREX1, KIAA1415 |
| Product description | The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.1) Lewis, J.P., et al. Genomics 96(4):211-219(2010) |
| Immunogen | This PREX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134-1163 amino acids from the C-terminal region of human PREX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PREX1 |
|---|---|
| Alternative Names | Phosphatidylinositol 3 5-trisphosphate-dependent Rac exchanger 1 protein P-Rex1 PtdIns(3 5)-dependent Rac exchanger 1 PREX1 KIAA1415 |
| Molecular Weight(MW) | 186 kDa |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
PREX1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
