WB
Various lysates were subjected to SDS PAGE followed by western blot with (PREX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PREX1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | PREX1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PREX1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000-1:8000 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | PREX1 |
|---|---|
| Gene Synonyms | P-REX1 |
| Gene Full Name | phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 |
| Gene Summary | The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008] |
| Alternative Names | KIAA1415 P REX1 Phosphatidylinositol 3 4 5-trisphosphate-dependent Rac exchanger 1 protein P-Rex1 PtdIns(3 4 5)-dependent Rac exchanger 1 |
| Molecular Weight(MW) | 186 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with (PREX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PREX1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:8000 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
