Application
Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 μg/mL.Application
Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 μg/mL.| Product Name | PRICKLE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRICKLE1 Antibody: RILP, EPM1B, RILP, Prickle-like protein 1 |
| Product description | PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.1) Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56 |
| Immunogen | PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1. The immunogen is located within the last 50 amino acids of PRICKLE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRICKLE1 |
|---|---|
| Alternative Names | PRICKLE1 Antibody: RILP EPM1B RILP Prickle-like protein 1 |
| Molecular Weight(MW) | Predicted: 46, 91 kDa Observed: 52, 90 kDa |
| Tissue Specificity | Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members. |
Application
Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 μg/mL.
Application
Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1310 - Human Bladder Tissue Lysate |
|---|---|
| Application Notes | PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRICKLE1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide. |
