Application
Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 μg/mL.Application
Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.| Product Name | PRICKLE2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRICKLE2 Antibody: EPM5, Prickle-like protein 2 |
| Product description | PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.1) Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56. |
| Immunogen | PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2. The immunogen is located within amino acids 420 - 470 of PRICKLE2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRICKLE2 |
|---|---|
| Alternative Names | PRICKLE2 Antibody: EPM5 Prickle-like protein 2 |
| Tissue Specificity | Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members. |
Application
Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 μg/mL.
Application
Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRICKLE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
