Application
Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 μg/mL.Application
Immunofluorescence of PRICKL4 in A549 cells with PRICKLE4 antibody at 20 μg/mL.Application
Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.| Product Name | PRICKLE4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRICKLE4 Antibody: OBTP, OEBT, TOMM6, C6orf49, Prickle-like protein 4, Overexpressed breast tumor protein |
| Product description | PRICKLE4 Antibody: PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.1) Teufel A, Weinmann A, Galle PR, et al. Characterization of OEBT, a LIM protein. Int. J. Mol. Med. 2005; 15:513-8. |
| Immunogen | PRICKLE4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PRICKLE4. The immunogen is located within the first 50 amino acids of PRICKLE4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRICKLE4 |
|---|---|
| Alternative Names | PRICKLE4 Antibody: OBTP OEBT TOMM6 C6orf49 Prickle-like protein 4 Overexpressed breast tumor protein |
| Sequence Similarities | 2) Cat. No. 17-003 - A549 Cell Slide |
| Tissue Specificity | Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members. |
Application
Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 μg/mL.
Application
Immunofluorescence of PRICKL4 in A549 cells with PRICKLE4 antibody at 20 μg/mL.
Application
Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRICKLE4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRICKLE4 Antibody is supplied in PBS containing 0.02% sodium azide. |
