Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | PRIM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA primase large subunit, 277-, DNA primase 58 kDa subunit, p58, PRIM2, PRIM2A |
| Product description | The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit. [provided by RefSeq].1) Vaithiyalingam, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13684-13689(2010) |
| Immunogen | This PRIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-345 amino acids from the Central region of human PRIM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRIM2 |
|---|---|
| Alternative Names | DNA primase large subunit 277- DNA primase 58 kDa subunit p58 PRIM2 PRIM2A |
| Molecular Weight(MW) | 59 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
