Application
Western blot analysis of lysates from Hela, HepG2, RD cell line (from left to right), using PRKAG3 Antibody at 1:1000 at each lane.| Product Name | PRKAG3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3, AMPKG3 |
| Product description | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq].1) Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010) |
| Immunogen | This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-454 amino acids from the C-terminal region of human PRKAG3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAG3 |
|---|---|
| Alternative Names | 5'-AMP-activated protein kinase subunit gamma-3 AMPK gamma3 AMPK subunit gamma-3 PRKAG3 AMPKG3 |
| Molecular Weight(MW) | 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of lysates from Hela, HepG2, RD cell line (from left to right), using PRKAG3 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
