Application
Immunohistochemistry staining of PRMT7 in kidney foramlin-fixed paraffin-embedded) tissue using PRMT7 Antibody.| Product Name | PRMT7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT7, KIAA1933 |
| Product description | Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. The PRMT proteins are recruited by C-terminal activation domain-2 of the SRC (steroid receptor coactivator) GRIP-1, whereas p300 interacts with activation domain-1 in the C terminus. have identified a mammalian arginine N-methyltransferase, PRMT7, that can catalyze the formation of omega-NG-monomethylarginine in peptides. In contrast to the other members of the PRMT family, PRMT7 was not able to methylate GST-fibrillarin or myelin basic protein or histone H2A, in vitro. It is also structurally different from other PRMTS in containing two putative AdoMet-binding motifs. Both of these domains are required for enzymatic activity. |
| Immunogen | PRMT7 antibody was raised against amino acids 346 - 360 of PRMT7 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| PRMT7 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PRMT7 |
|---|---|
| Alternative Names | PRMT7 KIAA1933 |
| Tissue Specificity | The amino acid sequence used as immunogen can be found in five different human PRMT7 isoforms: CRA_a (692 amino acids), CRA_b (613 amino acids), CRA_c (465 amino acids), CRA_d (655 amino acids) and CRA_e (567 amino acids). |
Application
Immunohistochemistry staining of PRMT7 in kidney foramlin-fixed paraffin-embedded) tissue using PRMT7 Antibody.| Application Notes | PRMT7 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | PRMT7 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
