Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PRMT7 using rabbit polyclonal PRMT7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).| Product Name | PRMT7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein arginine N-methyltransferase 7, 211-, Histone-arginine N-methyltransferase PRMT7, [Myelin basic protein]-arginine N-methyltransferase PRMT7, PRMT7, KIAA1933 |
| Product description | Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.1) Lee,J.H., et al. J. Biol. Chem. 280 (5), 3656-3664 (2005) |
| Immunogen | This PRMT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-692 amino acids from the C-terminal region of human PRMT7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PRMT7 |
|---|---|
| Alternative Names | Protein arginine N-methyltransferase 7 211- Histone-arginine N-methyltransferase PRMT7 [Myelin basic protein]-arginine N-methyltransferase PRMT7 PRMT7 KIAA1933 |
| Molecular Weight(MW) | 78 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PRMT7 using rabbit polyclonal PRMT7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
