PRMT8 probably methylates the guanidino nitrogens of arginyl residues in some proteins.1) Lee, J., (2005) J. Biol. Chem. 280 (38), 32890-32896.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT8.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PRMT8 antibody can be used for detection of PRMT8 by ELISA at 1:312500. PRMT8 antibody can be used for detection of PRMT8 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug HeLa lysate, Lane 2: 30ug HFF lysate, Lane 3: 30ug U2OS lysate).
Application
Antibody used in WB on Transfected 293T at 2.5 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
PRMT8 antibody can be used for detection of PRMT8 by ELISA at 1:312500. PRMT8 antibody can be used for detection of PRMT8 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PRMT8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt