Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of mouse Prodh Antibody in mouse liver, kidney tissue lysates (35ug/lane)Application
Western Blot at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution Lane 1: MDA-MB-468 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining PRODH in H. skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | PRODH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proline dehydrogenase 1, mitochondrial, Proline oxidase, Prodh, Pro1 |
| Product description | PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.1) Polyak,K., et.al., Nature 389 (6648), 300-305 (1997) Gogos,J.A., et.al., Nat. Genet. 21 (4), 434-439 (1999) |
| Immunogen | This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Prodh |
|---|---|
| Alternative Names | Proline dehydrogenase 1 mitochondrial Proline oxidase Prodh Pro1 |
| Molecular Weight(MW) | 68 kDa |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of mouse Prodh Antibody in mouse liver, kidney tissue lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution Lane 1: MDA-MB-468 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining PRODH in H. skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
