Application
Western blot of whole T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020 (500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120k PR-B isoform of the progesterone receptor phosphorylated at Ser294.| Product Name | Progesterone Receptor (phospho Ser294) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PR, NR3C3, Nuclear receptor subfamily 3 group C member 3, PR |
| Product description | There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.1) Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P., "Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody," Mol. Endocrinol., 14 (2000) 52 - 65. |
| Immunogen | Progesterone receptor (Ser294) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer294of human progesterone receptor. |
| Modification | p-Ser294 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PGR |
|---|---|
| Alternative Names | PR NR3C3 Nuclear receptor subfamily 3 group C member 3 PR |
| Molecular Weight(MW) | 90/120 |
| Tissue Specificity | Progesterone Receptor antibody is specific for the human progesterone receptor phosphorylated at Ser294. |
Application
Western blot of whole T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020 (500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120k PR-B isoform of the progesterone receptor phosphorylated at Ser294.| Application Notes | As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
