Application
Immunohistochemistry of Prosapip1 in rat brain tissue with Prosapip1 antibody at 2.5 μg/mL.Application
Immunofluorescence of Prosapip 1 in rat brain tissue with Prosapip 1 antibody at 20 μg/mL.Application
Western blot analysis of Prosapip1 in SK-N-SH cell lysate with Prosapip1 antibody at 1 μg/mL.| Product Name | Prosapip1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prosapip1 Antibody: PROSAPIP1, KIAA0552, PROSAPIP1, Leucine zipper putative tumor suppressor 3, ProSAP-interacting protein 1, ProSAPiP1 |
| Product description | Prosapip1 Antibody: Prosapip1 is a binding partner of the postsynaptic density (PSD) protein ProSAP2/Shank3, a major scaffolding protein in excitatory brain synapses. Prosapip1 is thought to bind ProSAP2/Shank3 through ProSAP2/Shank3's PDZ domain and link it in turn to the Spine-associated Rap-Gap (SPAR). Prosapip1 shares significant homology with another PSD protein, PSD-Zip70, with both coding for a Fez1 domain that can be found in a total of four related protein, forming a novel family of scaffolding molecules termed ‘Fezzins'. Recent studies show that Prosapip1 can be found in endocrine cells in which it is localized primarily in the hormone-storing compartment of these cells, suggesting that Prosapip1 may have other functions in non-neuronal cells.1) Wendholt D, Spilker C, Schmitt A, et al. ProSAP-interacting protein 1 (ProSAPiP1), a novel protein of the postsynaptic density that links the spine-associated Rap-Gap (SPAR) to the scaffolding protein proSAP2/Shank3. J. Biol. Chem.2006; 281:13805-16. |
| Immunogen | Prosapip1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Prosapip1. The immunogen is located within amino acids 590 - 640 of Prosapip1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Prosapip1 antibody can be used for detection of Prosapip1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ProSAPiP1 |
|---|---|
| Alternative Names | Prosapip1 Antibody: PROSAPIP1 KIAA0552 PROSAPIP1 Leucine zipper putative tumor suppressor 3 ProSAP-interacting protein 1 ProSAPiP1 |
Application
Immunohistochemistry of Prosapip1 in rat brain tissue with Prosapip1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Prosapip 1 in rat brain tissue with Prosapip 1 antibody at 20 μg/mL.
Application
Western blot analysis of Prosapip1 in SK-N-SH cell lysate with Prosapip1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | Prosapip1 antibody can be used for detection of Prosapip1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Prosapip1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Prosapip1 Antibody is supplied in PBS containing 0.02% sodium azide. |
