Application
Immunofluorescence of Prosapip 2 in rat liver tissue with Prosapip 2 antibody at 20 μg/mL.Application
Immunohistochemistry of Prosapip2 in rat liver tissue with Prosapip2 antibody at 5 μg/mL.Application
Western blot analysis of Prosapip2 in rat liver tissue lysate with Prosapip2 antibody at 1 μg/mL.| Product Name | Prosapip2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prosapip2 Antibody: SINTBAD, ProSAPiP2, KIAA0775, SINTBAD, TANK-binding kinase 1-binding protein 1, TBK1-binding protein 1 |
| Product description | Prosapip2 Antibody: Prosapip2 (TBKBP1) is essential for signal transduction during viral infection thus plays a major role in the TNF/NF-κB pathway. It is an adaptor protein that constitutively binds TBK1 (TANK-binding kinase) and IKBKE and may play a role in antiviral innate immunity. Prosapip2 is a 615 amino acid adaptor protein belonging to the fibrillar collagen family, consisting of trimers of identical alpha 1 chains which are linked to each other by interchain disulfide bonds. It has a ubiquitous expression with highest levels in ovary, followed by the neuronal system. Prosapip2 binds to TBK1 and helps in the activation of IRF3 which controls the expression of antiviral genes during infection. Recent studies show that Prosapip2 is an interaction partner of ProSAP2/Shank3 and actin, suggesting a role as a linker molecule between postsynaptic density and the cytoskeleton.1) Bouwmeester T, Bauch A, Ruffner H, et al. A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nat. Cell Biol.2004; 6:97-105 |
| Immunogen | Prosapip2 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Prosapip2. The immunogen is located within amino acids 500 - 550 of Prosapip2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Prosapip2 antibody can be used for detection of Prosapip2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBKBP1 |
|---|---|
| Alternative Names | Prosapip2 Antibody: SINTBAD ProSAPiP2 KIAA0775 SINTBAD TANK-binding kinase 1-binding protein 1 TBK1-binding protein 1 |
Application
Immunofluorescence of Prosapip 2 in rat liver tissue with Prosapip 2 antibody at 20 μg/mL.
Application
Immunohistochemistry of Prosapip2 in rat liver tissue with Prosapip2 antibody at 5 μg/mL.
Application
Western blot analysis of Prosapip2 in rat liver tissue lysate with Prosapip2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | Prosapip2 antibody can be used for detection of Prosapip2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Prosapip2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Prosapip2 Antibody is supplied in PBS containing 0.02% sodium azide. |
