Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate| Product Name | PRP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase PRP4 homolog, PRP4 kinase, PRP4 pre-mRNA-processing factor 4 homolog, PRPF4B, KIAA0536, PRP4, PRP4H, PRP4K |
| Product description | Pre-mRNA splicing occurs in two sequential transesterification steps, and PRP4 is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).1) Dellaire, G., et al., Mol. Cell. Biol. 22(14):5141-5156 (2002). |
| Immunogen | This PRP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 948-978 amino acids from the C-terminal region of human PRP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PRPF4B |
|---|---|
| Alternative Names | Serine/threonine-protein kinase PRP4 homolog PRP4 kinase PRP4 pre-mRNA-processing factor 4 homolog PRPF4B KIAA0536 PRP4 PRP4H PRP4K |
| Molecular Weight(MW) | 117 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
