Application
Immunocytochemistry of PPR5L in 293 cells with PPR5L antibody at 10 μg/mL.Application
Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptideApplication
Immunofluorescence of PPR5L in3T3 cells with PPR5L antibody at 20 μg/mL.| Product Name | PRR5L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRR5L Antibody: PROTOR2, PROTOR2, Proline-rich protein 5-like, Protein observed with Rictor-2, Protor-2 |
| Product description | PRR5L Antibody: Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.1) Thedieck K, Polak P, Kim ML, et al. 2007. PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. PLoS One 2007; 2:e1217. |
| Immunogen | PRR5L antibody was raised against a 17 amino acid synthetic peptide near the center of human PRR5L. The immunogen is located within amino acids 220 - 270 of PRR5L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRR5L |
|---|---|
| Alternative Names | PRR5L Antibody: PROTOR2 PROTOR2 Proline-rich protein 5-like Protein observed with Rictor-2 Protor-2 |
| Sequence Similarities | 3) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5. |
| Entrez Gene | 79899 |
|---|---|
| Protein Accession | NP_001153639 |
Application
Immunocytochemistry of PPR5L in 293 cells with PPR5L antibody at 10 μg/mL.
Application
Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Application
Immunofluorescence of PPR5L in3T3 cells with PPR5L antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRR5L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRR5L Antibody is supplied in PBS containing 0.02% sodium azide. |
