Application
Western blot analysis of PRRX1 in HeLa cell lysate with PRRX1 antibody at 1 μg/ml.| Product Name | PRRX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Paired related homeobox 1, homeobox protein PHOX1, Paired mesoderm homeobox protein 1, PHOX1, PMX1, PRX1, PRX-1 |
| Product description | The paired related homeobox protein 1 (PRRX1) is a paired-type DNA-binding homeodomain that plays an important role as a key switch for neural cell lineage determination and is essential for development (1,2). PRRX1 is expressed in a number of undifferentiated mesenchymal cells in mouse embryos and adults (3). Mice with a disrupted PRRX1 gene show perinatal death with craniofacial and limb malformations (4). Low PRRX1 expression levels were significantly associated with metastasis and poor prognosis of breast and colorectal cancer (5).1) Nohno T, Koyama E, Myokai F, et al. Chicken homeobox gene related to Drosophila paired is predominantly expressed in the developing limb. Dev. Biol. 1993; 158:254–64. |
| Immunogen | PRRX1 antibody was raised against a 19 amino acid peptide near the center of human PRRX1. The immunogen is located within amino acids 70 - 120 of PRRX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PRRX1 antibody can be used for detection of PRRX1 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRRX1 |
|---|---|
| Alternative Names | Paired related homeobox 1 homeobox protein PHOX1 Paired mesoderm homeobox protein 1 PHOX1 PMX1 PRX1 PRX-1 |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
| Tissue Specificity | PRRX1 antibody is human, mouse and rat reactive. At least two isoforms of PRRX1 are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of PRRX1 in HeLa cell lysate with PRRX1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | PRRX1 antibody can be used for detection of PRRX1 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRRX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PRRX1 antibody is supplied in PBS containing 0.02% sodium azide. |
