Application
Western blot analysis of PSMA7 Antibody in mouse spleen tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PSMA7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7, PSMA7, HSPC |
| Product description | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA7 is a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension.1) Seeger,M., et.al., J. Biol. Chem. 272 (13), 8145-8148 (1997) Huang,J., et.al., J. Virol. 70 (8), 5582-5591 (1996) |
| Immunogen | This PSMA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the Central region of human PSMA7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMA7 |
|---|---|
| Alternative Names | Proteasome subunit alpha type-7 Proteasome subunit RC6-1 Proteasome subunit XAPC7 PSMA7 HSPC |
| Molecular Weight(MW) | 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Western blot analysis of PSMA7 Antibody in mouse spleen tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
